Mushroom Spawn Production Technology（4）

While solid grain spawn remains a staple in mushroom cultivation, liquid spawn is rapidly gaining popularity in industrial-scale production due to its efficiency, uniformity, and faster colonization rates. Producing high-quality liquid spawn, however, requires specialized equipment that ensures consistent mycelial growth under sterile, oxygen-rich conditions. In this article, we’ll introduce the two main machines involved: the constant-temperature shaker and the liquid spawn fermenter.

1. Constant-Temperature Shaker (Incubator Shaker)

Function and Purpose

Also called a thermostatic oscillator or lab shaker, this device is used during the early phase of liquid spawn production. It gently agitates flasks containing nutrient-rich liquid culture, promoting even mycelial development.

Main Features

• Temperature Control
  Maintains a stable environment—usually around 24–28°C—ideal for mycelial growth.

• Oscillation Mechanism
  The shaking motion (either reciprocating or rotational) enhances oxygen transfer and prevents sedimentation of the mycelium.

• Capacity
  Suitable for culturing multiple flasks simultaneously, with adjustable speed and tray size.

Use Case

Ideal for producing mother liquid culture, which is then expanded in larger fermenters or used for direct inoculation of bags.

2. Liquid Spawn Fermenter

Overview

The liquid spawn fermenter is the core of modern liquid spawn production. It is a closed, automated system that allows for the large-scale cultivation of mycelium under sterile and controlled conditions.

Structure and Components

• Main Vessel
  Usually stainless steel, capable of holding dozens to hundreds of liters of culture.

• Aeration System
  Supplies filtered air to ensure adequate oxygen for mycelial respiration.

• Agitation Mechanism
  Mechanical stirring or air bubbling keeps the culture well-mixed.

• Temperature and pH Control
  Automated systems regulate internal conditions to promote optimal growth.

• Sterile Inlet/Outlet Ports
  Allow for aseptic transfer of culture fluids without contamination.

Advantages

• High Yield and Speed
  Produces large volumes of vigorous liquid spawn ready for use or expansion.

• Consistent Quality
  Controlled parameters minimize variation between batches.

• Ideal for Industrial Use
  Commonly used in spawn factories, mushroom bag production facilities, and integrated farms.

Is Liquid Spawn Right for You?

Liquid spawn production requires more technical expertise and a higher initial investment in equipment. However, the benefits in productivity and scalability make it an excellent choice for:

• Factories producing inoculated cultivation bags

• Operations with automated inoculation lines

• Growers seeking faster colonization and higher yields

Final Thoughts

Across this four-part series, we’ve explored the complete range of equipment required for professional mushroom spawn production—from sterilization to inoculation, incubation, and finally, liquid culture expansion. Whether you’re a mushroom farm, cooperative, or spawn supplier, choosing the right equipment lays the foundation for clean, consistent, and high-performing spawn.