Reishi Cultivation Technology（2）

Ganoderma lucidum cultivated on clinker has thick, wide caps, bright color, short cultivation cycle, high bioconversion rate and the best economic benefits. However, it is also restricted by forest resources and has certain limitations.
1. Cultivation Process
Section wood processing → bagging → sterilization → cooling → inoculation → management during germination period → soil preparation and bordering → covering with soil → management during germination period → harvesting.
2. Section Wood Processing
The tree species cultivated for Ganoderma lucidum are mainly hard broad-leaved tree species. The diameter of trees at breast height is preferably 5~15cm.
About a week before inoculation, cut the wood into 10~20cm long sections, and the cross section should be flat. Adjust the moisture content of the wood sections to 35%~42% by soaking in water or drying. Tie short pieces of wood of the same length into bundles with ropes and put them into plastic bags with matching specifications (polypropylene plastic bags or high-pressure-resistant polyethylene bags should be used for autoclaving).
3. Sterilization
Put the segmented wooden bag into a sterilization pot for sterilization and keep it at 100°C for 8 to 10 hours. After sterilization is completed, take it out of the pot when the temperature drops to 70°C. Move it into a clean and sterilized inoculation room or inoculation tent to cool down. When the temperature drops below 30°C, you can inoculate. Autoclave sterilize at 0.15MPa steam pressure for 2 hours. After natural cooling, move it into the inoculation room.
4. Inoculation
The sections can be inoculated when the temperature of the wood drops below 30°C. Use smoke disinfectant to disinfect the vaccination environment and vaccination tools before vaccination. Carry out the inoculation according to the aseptic operating procedures. Sprinkle the bacteria evenly on the upper surface of the wood sections and between the wood sections, and be sure to tie them tightly when tying them. After inoculation, it can be moved into the culture room for cultivation.

5.Management of Germination Period
The culture room has been strictly sterilized in advance, and after insecticide, the inoculated short-segment wood fungus bags are placed on the shelves. Leave a 70cm channel between shelves or between two bacteria walls to facilitate inspection. The temperature was controlled at 22~25°C and cultured in the dark. 7 days after inoculation, check the pile once. 15 to 20 days after inoculation, when a layer of bacterial skin forms on the surface of the wood section, the bag mouth can be opened to increase oxygen and reduce humidity. After the water droplets on the bag wall disappear, the bag mouth can be tightened and processed 3 to 4 times continuously to promote bacteria. The threads grow into the wood. After 45 to 55 days of culture, the mycelium will grow through the material, and after another 15 to 20 days of culture, it will reach physiological maturity. The general indoor cultivation period is about 60 days.
6. Preparing the Land and Making Borders
Plow the selected site 20cm deep to remove debris: make a border in an east-west direction. The border is 1.5~1.8m wide and 30cm deep, and the length depends on the site. Leave a walkway 35~70cm wide between the borders, and sprinkle lime on the bottom of the borders for disinfection. Dig drainage ditches around the site, with a depth of 30cm.
7. Cover Soil
Land covering should be carried out when the weather is fine. When the hyphae are physiologically mature and 1/3 of the fungus bags have mushroom buds, the fungus bags can be discharged to the ground. During this period, ensure that the greenhouse is well ventilated, provides a certain amount of scattered light, and avoids direct sunlight. The bags were opened and covered with soil 15 days after they were placed in the shed. The covering material requires loose and breathable sandy loam, and quicklime is added to adjust the pH to 8. Water thoroughly after covering with soil.
8. Management during the Production Period
After the Ganoderma lucidum segments are covered with soil, buds will appear in 8 to 12 days under suitable environmental conditions. At this time, the temperature inside the mushroom bed should be maintained at 26~28°C and the relative air humidity should be maintained at 80%~90% to promote cell differentiation on the mushroom surface. Reduce the number of ventilations to promote the elongation of the buds and the formation of stems. At the same time, scattered light with an illumination of 1000lx is given. When the Ganoderma stipe grows to 3~4cm, use a sharp knife to remove excess buds from the base, leaving 1 strong bud on each fungus stick.
When the stipe grows to 5~7cm long, the cap will be formed on the top of the stipe. At this time, reduce the number of water sprays, strengthen ventilation, reduce the relative humidity of the air, reduce the growth rate of the mushroom body, increase the density of the mushroom cap, and make the appearance of the mushroom body beautiful.
9. Harvest
When the cap of Ganoderma lucidum fruiting body no longer increases in size, the surface of the cap is uniform in color, and there is a curled ring with the same color as the cap on the edge. There are a large number of brown Ganoderma spores dispersed, and the color below the cap is consistent. It is ready for harvesting.