Reishi Cultivation Technology（3）

Ganoderma lucidum substitute cultivation includes bottle cultivation and bag cultivation. Except for the different culture containers, the two methods are almost the same in terms of culture medium formula, inoculation method and management measures.

1. Cultivation Process
Culture material selection → Mixing → Bagging → Sterilization → Cooling → Inoculation → Germination period management → Fruiting period management → Harvesting.

2. Determination of Cultivation Season
The production season arrangement has a close relationship with the output and quality of Lingyi substitute cultivation production. Properly arranged, the growth of fruiting bodies can obtain good natural growth conditions, and Ganoderma lucidum can grow well. Individual fruiting bodies are large and firm, with good quality and high yield. On the contrary, the fruiting bodies of Ganoderma lucidum are stunted. The suitable seasons for cultivation of Ganoderma lucidum in different regions vary due to different natural temperatures.

3.Cultivation Material Selection
The raw materials for Ganoderma cultivation are very wide, and broad-leaved tree chips, cottonseed husks, corn cobs, sugarcane bagasse, etc. can be used as the main materials. Wheat bran, rice bran, gypsum, lime, sucrose, superphosphate, etc. can be used as auxiliary materials. There are many cultivation formulas, and each place can adapt to local conditions and use local materials. The water content of the culture medium is 60%~65%, and the pH is 7.0~7.5.

4. Mixing
Use fresh, mildew-free culture media according to the formula. Use a mixer to mix the ingredients. First mix the main culture materials (such as cottonseed husks, corn cobs, sawdust, etc.) evenly, then add other raw materials and mix evenly.

5. Bagging
Plastic bags commonly used for bag planting of Ganoderma lucidum include polypropylene or low-pressure polyethylene bags. Commonly used bags are 17cm×35cm or 20cm×45cm and 0.004cm thick. Mechanically or manually bagged, filled and compacted. Punch a 2cm thick hole in the middle to the bottom of the material and seal it.

6. Sterilization
The filled bags should be sterilized in time, and the sterilization temperature should be maintained at 100°C for 8 to 10 hours, or sterilized by high pressure at 0.15MPa for 2 hours. After sterilization, move the material bag into a clean cooling room and inoculate it when the temperature of the material is lower than 28°C.

7. Inoculation
After the bag temperature is lower than 28°C, inoculate in the inoculation room according to aseptic operation. Insert the bacteria into the hole and seal it. Use one end of a 17cm×35cm, 0.004cm thick plastic bag to inoculate. The inoculation volume is generally 20 bags of 500mL strain bottles, or branch strains are inserted. Inoculate both ends of a 20cm×45cm, 0.004cm thick plastic bag. The inoculation volume is generally 10 bags of 500ml strain bottles.

8.Management of the Fungus Development Period
After the culture room is cleaned and disinfected, move the inoculated bacteria bags to the shelves in the culture room. 2 to 3 days after inoculation, the temperature of the culture room is controlled at 25 to 26°C to promote mycelium colonization. It is not appropriate to turn or ventilate at this time. 4 to 5 days after inoculation, colonies will form and the temperature can drop to 22 to 24°C. Pay attention to ventilation and control the relative humidity of the air at 60% to 65%. 15~20 days after inoculation, the mycelium eats 4~5cm inwards. At this time, the temperature is controlled at 20~22℃, and attention is paid to ventilation. After 25 to 35 days of culture, the hyphae will cover the fungus bag. After another 7 to 10 days of culture, the mycelium reaches post-maturity. During the cultivation process, the pile was turned over every 10 to 15 days. In the later stage of bacterial growth, the ventilation volume can be increased and 100~200lx scattered light can be given. When the mycelium differentiates into primordia individually, it indicates that the mycelium has reached physiological maturity and can be moved into the mushroom shed for mushroom management.