Shimeji Mushroom Cultivation Technology（3）

1. Scratching bacteria and filling with water
(1) Bottle Growth
After the fungi of shimeiji mushroom reaches physiological maturity, it can be infested with bacteria. After scratching, immediately inject 10~20mL of water to replenish the moisture on the surface of the material and increase the number of primordia formed.
(2) Bag Growth
After scratching the physiologically mature fungi bag, you can inject clean water into the bag and keep it for 1 hour. Then pour out the absorbed water. Be careful to pour the water cleanly to avoid the generation of miscellaneous bacteria.

2. Promote Budding
After scratching the fungi and injecting water, dark management is carried out for 4~7 days. After the fungi resumes growth, it enters the primordium differentiation stage. At this time, the temperature of the mushroom induction room is controlled at 14~15℃. The budding stage requires a higher relative humidity of the air. Usually, an ultrasonic humidifier is used to produce atomized water to control the air humidity at 95%~98%. The primordium differentiation stage requires an illumination of 100lx and more than 10 hours of light per day. The carbon dioxide concentration is controlled at 1000~2000μL/L, which is conducive to primordium differentiation. The carbon dioxide concentration is controlled by adjusting the ventilation time and ventilation volume. Under suitable conditions, 7~8 days after scratching the mushrooms, the surface of the culture medium will show tumor-like protrusions, and piles of small granular white mushroom buds will appear.

3. Mushroom Cultivation Period
After 2-3 days of primordium differentiation, the small granular mushroom buds gradually grow into a thin and thick stipe at the top, and a hemispherical cap is differentiated at the top.
(1) Temperature
The temperature during the fruiting body growth period is controlled at 14-15℃.
(2) Relative humidity of the air
Controlled at 90%-95%. If the humidity is insufficient, the mushroom will die; if the humidity is too high, the fruiting body will grow slowly, the stipe will turn black, and it will be easily attacked by diseases.
(3) Carbon dioxide concentration
Ventilate 5-7 times a day, 15 minutes each time, and maintain a carbon dioxide concentration of about 2000μL/L. When the mushroom cap grows to a diameter of 0.5-0.8cm, reduce ventilation and increase the carbon dioxide concentration to 3000μL/L to inhibit the growth of the mushroom cap and promote the elongation and thickening of the stipe.
(4)Lighting
The growth of the fruiting body of the mushroom requires sufficient light. The mushroom house requires about 500lx of light, with more than 10 hours of light per day.
The factory-produced cultivation bottles can be dug out and processed after harvesting. The waste can be reused or processed into waste and fuel for reuse.