Supplementary ingredients play a crucial role in enhancing the nutritional composition of mushroom substrates, supporting mycelium growth, and improving yield. The following materials are commonly used as supplementary ingredients, with only one being necessary per substrate formulation.
Also known as wheat husk or wheat middlings, wheat bran is a byproduct of wheat milling. It is yellow, flaky, or powdery and rich in B vitamins, making it a valuable nitrogen source. With low oil content, wheat bran resists oxidation, ensuring stability in mushroom production. Its usage and precautions are similar to rice bran.
A residue obtained from soybean oil extraction, soybean meal is more than three times richer in nitrogen than high-quality wheat bran. Its price fluctuates due to various factors, and some mushroom farms replace wheat bran with soybean meal to maintain stable nitrogen content while reducing transportation costs. It is typically ground into fine powder for efficient utilization.
Ground corn kernels passing through a 20-mesh sieve with no more than 10% coarse particles, corn flour is light yellow and slightly sweet, with moisture content below 14%. It contains 9.6% crude protein, 5.6% crude fat, 1.5% crude fiber, 69.7% nitrogen-free extract, and 1.0% crude ash, with a carbon content of 46.7% and nitrogen content of 2.3%. Rich in biotin (vitamin H), corn flour slows down mycelial aging and is often used as a yield enhancer at 2%-3% of the substrate mixture.
Also known as fine bran or green bran, rice bran serves as both a carbon and nitrogen source, directly accessible to mycelium. High-quality rice bran should be fresh, free of off-odors, aromatic, and smooth to the touch. It should contain over 12.5% crude protein, less than 13% moisture, and be free of impurities. Due to its germ content, the particle size standard requires no more than 10% exceeding 20-mesh. Light yellow and slightly sweet, rice bran enables rapid nutrient absorption for certain mushroom species that struggle to extract nutrients from sawdust. For long-cycle species like Lion’s Mane and White Beech mushrooms, sawdust is added to extend the growth cycle, ensuring texture, quality, and yield.
Soybean hulls, a byproduct of soybean processing, contain approximately 12.2% crude protein and 38% crude fiber, with low lignin content (around 2%). Rich in vitamins C, E, and B, as well as minerals like phosphorus, iron, zinc, calcium, and magnesium, soybean hulls are widely used as a protein supplement in mushroom cultivation. Beet pulp, a byproduct of sugar production, has high water content and is dried for market supply. Despite its high cost, its excellent water retention capacity (up to 600%) makes it valuable for increasing substrate moisture.
A low-cost, high-nutrient byproduct of soybean processing, dried bean dregs consist of soybean hulls and ground residues. With an absorption rate of up to 600%, they serve as an effective water-retaining supplement.
Composed of grain husks, leaf buds, insoluble proteins, hemicellulose, fats, ash, and trace amounts of undecomposed starch, distiller’s grains contain 25.13% crude protein, 7.13% crude fat, 13.81% crude fiber, 3.64% ash, 0.4% calcium, and 0.57% phosphorus. They are also rich in essential trace elements like manganese, iron, and copper.
These supplementary ingredients enhance mushroom substrate nutrition, promoting faster colonization and increased yields. Selection depends on substrate composition, mushroom species, and local availability, ensuring optimal production efficiency.